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Tn5-based chromatin footprinting and regulatory motif analysis

fp-tools provides command-line and browser workflows for bulk and single-cell footprinting of Tn5-based chromatin profiling data, including ATAC-seq, CUT&Tag, and related assays. It connects bias correction, footprint scoring, motif analysis, replicate comparisons, and reusable reports.

PyPI GitHub Python 3.11–3.13 MIT license
  • Analyze aligned Tn5-based chromatin data from BAM and peak files.
  • Compare motif-associated footprint scores across samples and replicates.
  • Analyze pseudobulk and per-cell footprint signatures.
  • Export static figures and portable interactive HTML reports.

Choose your starting point:

Linux command-line and container users can optionally prepare FASTQ files with prepare-atac.

Install fp-tools  ·  View the tool overview